Subject Of Case Study Written: This is the paper of our study on annealing-induced fiber growth. This study is part of our studies on fiber growth arising out of the inversion of the normal strain of annealing, annealing-induced fiber growth. Competing interests =================== The authors declare no conflict of interest. Authors\’ contributions ======================= WW participated in conception, design and data acquisition, data analysis, and wrote the paper. TWV, RB, MW, SL, and YW performed mechanical measurements. QAH carried out microsurgic measurements. NAE performed experimental and experimental analyses and helped to draft the manuscript. NAE intended to carry out fiber growth modelling. DRI, DPH, CM, CMM, and FF performed statistical analyses of experimental data. NAE and FW designed the study.
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 Calcium used as a substrate in case study (Schematic illustration). (2) Fibers were considered as fiber tissue in Figure 1A. The initial fiber diameter is in D5, and gradually changes into various smaller diameter (D5/D10, D10/D55, D55/D60, and D100/D100) in phase before degradation.](S  When the fiber diameter is at D5, X and radius of D5 are the original diameter and radius, respectively, while the Y position corresponds to the location of newly formed fiber.](S  Calcium used as a substrate in case study (Schematic illustration). (2) Fibers were considered as fiber tissue in Figure 1B. Fibers were in the y axis, membrane-based, or flexible ([Fig. 1](#F1){ref-type=”fig”}), depending on density, for a one-in-one-out fiber-proliferation model (*n* = 3). Fibers were subjected to a compression force of 30 M/ 50, 100 M/ 50, 400 M/ 50 and 1,000 µm/100 µm. Due to the fact that growth process through the die was altered, the growth process was described by the same mechanism that happens in later experiments when growth is done to various diameter ranges of fiber diameter.
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(3) The parameters of the fiber model. It can be seen the fiber diameter to each of the two maximum diameter ranges in [Fig. 1](#F1){ref-type=”fig”}; **a**) Deformability, **b**) Deformability from cell viability, **c**) Deformability from cell viability. Finally, **d**) Fiber deformation (c) which shows the changes of fiber diameter and cell viability.](S 








